protein extraction reagent (Boster Bio)

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Boster Bio protein extraction reagent
Protein Extraction Reagent, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+extraction+reagent/Plus+RIPA+Lysis+Buffer/pm41921865-118-6-10
Average 95 stars, based on 57 article reviews

protein extraction reagent - by Bioz Stars, 2026-10

95/100 stars

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Protein Extraction:

Article Title: Circ-Ntrk2 acts as a miR-296-5p sponge to activate the TGF-β1/p38 MAPK pathway and promote pulmonary hypertension and vascular remodelling.
Article Snippet: The fluorescence microscope (Leica DMi8, Wetzlar, Germany) was used to capture fluorescent images. .. Lung tissues were homogenised with a Protein Extraction Reagent (BOSTER, Wuhan, China) using an automatic homogeniser (FastPrep-24 5G, MP Biomedicals, Santa Ana, CA, USA). .. Supernatants were collected after the homogenates were centrifuged (12,000 rpm, 4 °C) for 30 min. PASMC proteins were extracted with Protein Extraction Reagent (BOSTER).

Article Title: METTL3 Plays Regulatory Roles in Acute Pneumonia during Staphylococcus aureus Infection.
Article Snippet: Pneumonia caused by Staphylococcus aureus infection has consistently been a significant cause of morbidity and mortality worldwide.. Extensive research to date indicates that N6-methyladenosine (m6A) modification plays a crucial role in the development and progression of various diseases.. However, it remains unknown whether the m6A modification affects the progression of bacterial pneumonia.

Article Title: Beneficial effects of electro-acupuncture treatment in photothrombotic ischemia model rats by remodeling neurovascular unit.
Article Snippet: .. Total proteins were extracted using a protein extraction reagent (AR0102, Boster Biological Technology), and protein concentrations were quantified with a BCA protein assay kit (AR0198, Boster Biological Technology). ..

Article Title: Mechanistic study of Nidus Vespae inhibiting gastric cancer in vitro through the JAK2/STAT3 signaling pathway.
Article Snippet: .. For the isolation of total proteins, a protein extraction reagent (Boster, Wuhan, China) was utilized, which contains a cocktail of protease and phosphatase inhibitors (AbMole, Houston, USA). .. The concentrations of the total proteins were subsequently quantified employing the Bradford Protein Assay Kit (Beyotime, Shanghai, China).

Article Title: Hepatocyte‐Derived FGF1 Alleviates Isoniazid and Rifampicin‐Induced Liver Injury by Regulating HNF4 α ‐Mediated Bile Acids Synthesis
Article Snippet: .. Total protein from liver homogenates or cells (HepG2 cell or primary hepatocyte) was extracted with protein extraction reagent (Boster Bioengineering), and nuclear proteins were extracted with Nuclear and Cytoplasmic Protein Extraction Kit (KeyGen BioTech). .. After the protein concentration in the supernatants was measured using Coomassie brilliant blue method, the protein samples were mixed with protein loading buffer (Fudebio‐tech), then denatured at 100 °C for 10 min.

Article Title: Hepatocyte-Derived FGF1 Alleviates Isoniazid and Rifampicin-Induced Liver Injury by Regulating HNF4α-Mediated Bile Acids Synthesis.
Article Snippet: .. Western Blotting Analysis: Total protein from liver homogenates or cells (HepG2 cell or primary hepatocyte) was extracted with protein extraction reagent (Boster Bioengineering), and nuclear proteins were extracted with Nuclear and Cytoplasmic Protein Extraction Kit (KeyGen BioTech). .. After the protein concentration in the supernatants was measured using Coomassie brilliant blue method, the protein samples were mixed with protein loading buffer (Fudebio-tech), then denatured at 100 °C for 10 min.

Article Title: Cross-talk between NOTCH2 and BMP4/SMAD signaling pathways in bovine follicular granulosa cells.
Article Snippet: NOTCH and bone morphogenetic protein (BMP)/SMAD signaling play key regulatory roles in mammalian ovarian development.. The study aimed to investigate interregulatory mechanisms between NOTCH2 and BMP4/SMAD signaling pathways in bovine follicular granulosa cells (GCs).. The results showed that NOTCH2 silence reduced the mRNA expression of SMAD1, SMAD5, SMAD8 (also known as SMAD9) and Mg2þ/Mn2þdependent Protein Phosphatase 1A (PPM1A), which are effectors of BMP/SMAD signaling pathway (P < 0.01).

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Article Title: Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway.
Article Snippet: Acute lung injury (ALI) is a common lung disease characterized by severe acute inflammatory lung injury in patients with sepsis.. Syringaresinol (SYR) has been reported to have anti-apoptotic and anti-inflammatory effects, but whether it could prevent pyroptosis to improve sepsis-induced ALI remains unclear.. The purpose of this work was to examine the impact of SYR on sepsis-induced ALI and investigate the underlying mechanisms.

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